Anti-EPHA2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	P29317
Gene Id	1969
Gene Symbols	EPHA2
Protein Name / Synonyms	EPHA2, ARCC2, CTPA, CTPP1, CTRCT6, ECK, EPH receptor A2
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

54kDa/108kDa

Observed MW

90kDa

Immunogen

A synthetic phosphorylated peptide around Y594 of human EphA2 (NP_004422.2).

Modification

Phospho Y594

Storage

-20°C

Images

Western blot analysis of extracts of SH-SY5Y cells, using Phospho-EphA2-Y594 antibody (144-61534) at 1:1000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/ml) for 5 minutes .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - Phospho-EphA2-Y594 pAb (144-61534)

Expiration:

12 months from the date of shipment when stored properly.

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