Anti-EphB1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P54762
Gene Id	2047
Gene Symbols	EPHB1
Protein Name / Synonyms	EphB1 (C-term)
Clonality	Polyclonal
Preparation	This EphB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-984 amino acids from the C-terminal region of human EphB1.
Concentration (lot specific)	1.200 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Specificity

Other Names

EPHB1; ELK; EPHT2; HEK6; NET; Ephrin type-B receptor 1; EPH tyrosine kinase 2; EPH-like kinase 6; Neuronally-expressed EPH-related tyrosine kinase; Tyrosine-protein kinase receptor EPH-2

NCBI Accession #

NP_004432.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

109885

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-EphB1 Pab (Cat# 102-17470) in mouse brain tissue. EphB1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d Loss of EphB1 expression in gastric cancer cells.(Provided by Jian-dong Wang,Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine)
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	EPHB1 Antibody (H970) (Cat# 102-17470) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the EPHB1 antibody detected the EPHB1 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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