Anti-EPS8 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	2059
Protein Name / Synonyms	EPS8, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

EPS8 antibody, epidermal growth factor receptor kinase substrate 8 antibody, epidermal growth factor receptor pathway substrate 8 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

92 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 761 and 822 of EPS8 (Uniprot ID# Q12929)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EPS8 antibody [C3], C-term (107-10199) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
EPS8 antibody
107-10199 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products