Anti-ERBB2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04626
Gene Id	2064
Gene Symbols	ERBB2
Protein Name / Synonyms	ERBB2(N-term)
Clonality	Polyclonal
Preparation	This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human ERBB2.
Concentration (lot specific)	2 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis, Cardiovascular Disease, Cancer, Tyrosine Kinase Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An Interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen=CD340; Flags: Precursor

NCBI Accession #

NP_001005862.1;NP_004439.2

Other Accession #

P06494; P70424

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

137910

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:50-100

	Western blot analysis of ErbB2 (arrow) in T47D cell lysates, either noninduced (Lane 1) or induced with HRG (Lane 2).
	Flow cytometric analysis of MCF-7 cells using HER2/ErbB2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HER2/ErbB2 antibody (N-term) (Cat# 102-17484), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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