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Anti-ERH Antibody
Catalog #: 107-10291
ERH antibody, 0.79 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 2079 |
| Protein Name / Synonyms | ERH |
| Concentration (lot specific) | 0.79 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
DROER antibody, FLJ27340 antibody, ERH antibody, enhancer of rudimentary homolog antibody, enhancer of rudimentary homolog (Drosophila) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
12 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 of ERH
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
ERH antibody detects ERH protein at cytosol on Breast Carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Breast Carcinoma.
ERH antibody (107-10291) dilution: 1:500.
Sample: Paraffin-embedded Breast Carcinoma.
ERH antibody (107-10291) dilution: 1:500.
ERH antibody detects ERH protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ERH antibody (107-10291) dilution: 1:500.
Sample: Paraffin-embedded mouse fore brain.
ERH antibody (107-10291) dilution: 1:500.
ERH antibody detects ERH protein by western blot analysis.
A. 30 µg A549 whole cell extract
B. 30 µg H1299 whole cell extract
C. 30 µg HCT116 whole cell extract
15 % SDS-PAGE
ERH antibody (107-10291) dilution: 1:500
A. 30 µg A549 whole cell extract
B. 30 µg H1299 whole cell extract
C. 30 µg HCT116 whole cell extract
15 % SDS-PAGE
ERH antibody (107-10291) dilution: 1:500
Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
107-10291 diluted at 1:1000
A: mouse brain
15% SDS PAGE
107-10291 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.