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Anti-ERK (p202/204) (Phospho) Antibody, Biotinylated
Catalog #: RB-02-0019B-50
Rabbit anti-ERK (p202/204) (Phosph) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P28482 |
| Gene Id | 5594 |
| Gene Symbols | MAPK1 ERK2 PRKM1 PRKM2 |
| Clonality | Polyclonal |
| Immunogen | The imunogen was synthetic peptide. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CTGFLT(p)EY(P)VA |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:2, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease, Cancer, MAPK Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
ERK is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat ERK(p202/204)(Phosph) has not yet been tested.
Related Products
- Goat Anti-HumanERK1 / MAPK3, N-terminus (catalog #: DS-PB-02257)
- RayBio® Cell-Based ELISA for detecting ERK1 / 2 (Thr202/Tyr204) in cultured cell line (catalog #: CBEL-ERK-1)
References
- Xue A, et al.. (2009) Suppression of urokinase plasminogen activator receptor inhibits proliferation and migration of pancreatic adenocarcinoma cells via regulation of ERK/p38 signaling. Int J Biochem Cell Biol. 41(8-9):1731-8.
- Coles LS,et al..(2005) Phosphorylation of cold shock domain/Y-box proteins by ERK2 and GSK3beta and repression of the human VEGF promoter. FEBS Lett. 579(24):5372-8.
Expiration:
12 months from the date of shipment when stored properly.