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Anti-ERK (p202/204) (Phospho) Antibody
Catalog #: RB-02-0019
Rabbit anti-Human ERK (p202/204) (Phosph) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat ERK(p202/204)(Phosph) has not yet been tested. |
| Accession Number | P28482 |
| Gene Id | 5594 |
| Gene Symbols | MAPK1 ERK2 PRKM1 PRKM2 |
| Clonality | Polyclonal |
| Immunogen | The imunogen was synthetic peptide. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CTGFLT(p)EY(P)VA |
| Concentration (lot specific) | 1.250 mg/ml |
| Recommended Applications | ELISA (recommended work dilution= 1:2, 000) |
| Reconstitution | Product is supplied as a solution obtained from purified antibody in PBS (pH 7.5) with 50% glycerol without preservatives. |
| Research Area | Cardiovascular Disease, Cancer, MAPK Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
ERK is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Typical Data
Storage/Stability
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid multiple freeze-thaw cycles, as this will lower the activity of the antibody.
Expiration
12 months from the date of shipment when stored properly.
References
- Xue A, et al.. (2009) Suppression of urokinase plasminogen activator receptor inhibits proliferation and migration of pancreatic adenocarcinoma cells via regulation of ERK/p38 signaling. Int J Biochem Cell Biol. 41(8-9):1731-8.
- Coles LS,et al..(2005) Phosphorylation of cold shock domain/Y-box proteins by ERK2 and GSK3beta and repression of the human VEGF promoter. FEBS Lett. 579(24):5372-8.