Anti-ERK4 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P31152
Gene Id	5596
Gene Symbols	MAPK4
Protein Name / Synonyms	ERK4 (C-term)
Clonality	Polyclonal
Preparation	This ERK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-557 amino acids from the C-terminal region of human ERK4.
Concentration (lot specific)	1.640 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The CMGC group consists of 60 kinases including the cyclin-dependent kinase (CDK) and close relatives family, the MAP kinase (ERK) family, the glycogen synthase kinase 3 (GSK3) family, and the Cdc2-like kinase (CLK) family.

Specificity

Other Names

MAPK4; ERK4; PRKM4; Mitogen-activated protein kinase 4; Extracellular signal-regulated kinase 4; MAP kinase isoform p63

NCBI Accession #

NP_002738.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

65922

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of anti-ERK4 Pab (Cat# 102-17390) in mouse brain tissue lysate. ERK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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