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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 1999 |
| Protein Name / Synonyms | ESE1, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
EPR-1 antibody, ERT antibody, ESE-1 antibody, ESX antibody, ELF3 antibody, "ets domain transcription factor, serine box (epithelial-specific) antibody", "E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific) antibody", epithelium-restricted Ets protein ESX antibody, E74-like factor 3 (ets domain transcription factor) antibody, epithelial-restricted with serine box antibody, ETS-related transcription factor Elf-3 antibody, epithelium-specific Ets transcription factor 1 antibody, "E74-like factor 3 (ets domain transcription factor, epithelial-specific ) antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
41 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 309 and 371 of ESE1 (Uniprot ID# P78545)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using ESE1(107-10197) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ESE1 (107-10197) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-10197 diluted at 1:1000
A: A431
10% SDS PAGE
107-10197 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.