Anti-ESRRA (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P11474
Gene Id	2101
Gene Symbols	ESRRA
Protein Name / Synonyms	ESRRA (Center)
Clonality	Polyclonal
Preparation	This ESRRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human ESRRA.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ESRRA is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

ESRRA; ERR1; ESRL1; NR3B1; Steroid hormone receptor ERR1; Estrogen receptor-like 1; Estrogen-related receptor alpha; Nuclear receptor subfamily 3 group B member 1

NCBI Accession #

NP_001269379.1;NP_001269380.1;NP_004442.3

Other Accession #

P62510; P62509; P62508; P11475; Q61539; O95718; Q5QJV7; O08580

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

45510

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of ESRRA Antibody (Center) (Cat# 102-17958) in A375 cell line lysates (35ug/lane). ESRRA (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of 293 cells using ESRRA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody (Center) (Cat# 102-17958). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.
	Confocal immunofluorescent analysis of ESRRA Antibody (Center) (Cat# 102-17958) with 293 cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).