ETEA antibody [C3], C-Terminus, 0.57 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 23197 |
| Protein Name / Synonyms | ETEA, C-term |
| Concentration (lot specific) | 0.57 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ETEA antibody, KIAA0887 antibody, UBXD8 antibody, UBXN3B antibody, FAF2 antibody, UBX domain protein 3B antibody, UBX domain containing 8 antibody, UBX domain-containing protein 8 antibody, expressed in T-cells and eosinophils in atopic dermatitis antibody, UBX domain-containing protein 3B antibody, FAS-associated factor 2 antibody, Fas associated factor family member 2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
53 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 383 and 445 of ETEA (Uniprot ID# Q96CS3)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
ETEA antibody [C3], C-term detects FAF2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAF2 protein stained by ETEA antibody [C3], C-term (107-10723) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAF2 protein stained by ETEA antibody [C3], C-term (107-10723) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using ETEA(107-10723) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
107-10723 diluted at 1:1000
A: H1299
B: Hela
10% SDS PAGE
107-10723 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.