Anti-FAN (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	8439
Protein Name / Synonyms	FAN, Internal
Concentration (lot specific)	0.93 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FAN antibody, NSMAF antibody, protein FAN antibody, factor associated with N-SMase activation antibody, factor associated with neutral sphingomyelinase activation antibody, neutral sphingomyelinase (N-SMase) activation associated factor antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

104 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 262 and 570 of FAN (Uniprot ID# Q92636)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded T(gastric CA) , using NSMAF(107-10400) antibody at 1:100 dilution.

Western Blot analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody.
A: Non-transfected lysate.
B: NSMAF transfected lysate.
7.5% SDS PAGE
107-10400 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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