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Anti-FANCC Antibody

Catalog #: 107-10222

FANCC antibody [N1C1], 0.99 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Gene Id
2176
Protein Name / Synonyms
FANCC
Concentration (lot specific)
0.99 mg/ml
Recommended ApplicationsICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

FA3 antibody, FAC antibody, FACC antibody, FLJ14675 antibody, FANCC antibody, Fanconi anemia group C protein antibody, "Fanconi anemia, complementation group C antibody"

Specificity

Species Reactivity
Human, Mouse

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
63 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 305 of FANCC (Uniprot ID# Q00597)

Applications

Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using FANCC(107-10222) antibody at 1:100 dilution.
FANCC antibody detects FANCC protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5 % SDS-PAGE, and blotted with FANCC antibody (107-10222) diluted by 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FANCC(107-10222) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)

A: NIH-3T3

7.5% SDS PAGE

FANCC antibody

107-10222 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.