Anti-FAS (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P25445
Gene Id	355
Gene Symbols	FAS
Protein Name / Synonyms	FAS (Center)
Clonality	Polyclonal
Preparation	This FAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the Central region of human FAS.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Apoptosis, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.

Specificity

Other Names

FAS; APT1; FAS1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD_antigen=CD95; Flags: Precursor

NCBI Accession #

NP_000034.1;NP_690610.1;NP_690611.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

37732

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	Western blot analysis of FAS Antibody (Center) (Cat# 102-17903) in T47D cell line lysates (35ug/lane).FAS (arrow) was detected using the purified Pab.
	Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (Center) (Cat# 102-17903). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.
	Confocal immunofluorescent analysis of FAS Antibody (Center)(Cat# 102-17903) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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