3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-FAST Antibody
Catalog #: 107-10344
FAST antibody, 6.56 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 10922 |
| Protein Name / Synonyms | FAST |
| Concentration (lot specific) | 6.56 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FAST antibody, FASTK antibody, FAST kinase antibody, fas-activated serine/threonine kinase antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 346 and 549 of FAST (Uniprot ID# Q14296)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
FAST antibody detects FAST protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FAST protein stained by FAST antibody (107-10344) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FAST protein stained by FAST antibody (107-10344) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST(107-10344) antibody at 1:100 dilution.
FAST antibody detects FASTK protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (107-10344) dilution: 1:1000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (107-10344) dilution: 1:1000
FAST antibody detects FASTK protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (107-10344) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (107-10344) dilution: 1:1000
Immunofluorescence analysis of methanol-fixed HeLa, using FAST (107-10344) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
107-10344 diluted at 1:1000
A:NIH-3T3
7.5% SDS PAGE
107-10344 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.