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Anti-Fatty Acid Synthase (N-term) Antibody
Catalog #: 107-10915
Fatty Acid Synthase antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 2194 |
| Protein Name / Synonyms | Fatty Acid Synthase, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Cardiovascular Disease, Glycolysis & Lipid Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FAS antibody, MGC14367 antibody, MGC15706 antibody, OA-519 antibody, SDR27X1 antibody, FASN antibody, "short chain dehydrogenase/reductase family 27X, member 1 antibody", fatty acid synthase antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
273 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 401 and 694 of Fatty Acid Synthase (Uniprot ID# P49327)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:2000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase(107-10915) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1N2], N-term (107-10915) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1N2], N-term (107-10915) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (50 ug of whole cell lysate)
A: mouse liver
5% SDS PAGE
107-10915 diluted at 1:1000
A: mouse liver
5% SDS PAGE
107-10915 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded OECM1 xenograft, using Fatty Acid Synthase(107-10915) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
5% SDS PAGE
107-10915 diluted at 1:1000
A: Molt-4
5% SDS PAGE
107-10915 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.