Synonyms
FLJ20188 antibody, Fbl12 antibody, FBXL12 antibody, F-box/LRR-repeat protein 12 antibody, F-box protein FBL12 antibody, F-box and leucine-rich repeat protein 12 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
37 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 265 and 326 of FBXL12 (Uniprot ID# Q9NXK8)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL12(107-10494) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
107-10494 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
FBXL12 antibody
107-10494 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.