Anti-FDPS (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P14324
Gene Id	2224
Gene Symbols	FDPS
Protein Name / Synonyms	FDPS (N-term)
Clonality	Polyclonal
Preparation	This FDPS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-112 amino acids from the N-terminal region of human FDPS.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IHC-P, Western Blotting, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The isoprene biosynthetic pathway supply the cell with cholesterol, ubiquinone, and various nonsterol metabolites. The farnesylpyrophosphate synthetase enzyme catalyzes the formation of geranyl and farnesylpyrophosphate from isopentenylpyrophosphate and dimethylallyl pyrophosphate. Analysis of FDPS activity and protein in rat liver, accompanied by immunofluorescence and immunoelectron microscopy studies, demonstrated that FDPS is predominantly localized in peroxisomes.1 Liver tissue from patients with the peroxisomal deficiency diseases Zellweger syndrome and neonatal adrenoleukodystrophy exhibit diminished activities of FDPS and subsequent isoprenoid synthesis.

Specificity

Other Names

FDPS; FPS; KIAA1293; Farnesyl pyrophosphate synthase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Geranyltranstransferase

NCBI Accession #

NP_001129293.1;NP_001129294.1;NP_001229753.1;NP_001229754.1;NP_001995.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48275

Recommended Dilutions

IF: 1:100; IHC: 1:50-100; WB: 1:1000

	Immunofluorescent analysis of A549 cells, using FDPS Antibody (N-term) (Cat# 102-15033). 102-15033 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor^® 554 (red) conjugated Phalloidin (red).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Western blot analysis of FDPS (arrow) using rabbit polyclonal FDPS Antibody (D31) (Cat# 102-15033). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FDPS gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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