Anti-FEN1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P39748
Gene Id	2237
Gene Symbols	FEN1
Protein Name / Synonyms	FEN1 (Center)
Clonality	Polyclonal
Preparation	This FEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human FEN1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. This protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Specificity

Predicted Reactivity

Bovine

Other Names

FEN1; RAD2; Flap endonuclease 1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1

NCBI Accession #

NP_004102.1

Other Accession #

Q58DH8

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42593

Recommended Dilutions

IF: 1:100; WB: 1:1000

	Immunofluorescent analysis of U251 cells, using FEN1 Antibody (Center) (Cat# 102-15185). 102-15185 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
	Western blot analysis of anti-FEN1 Antibody (Center) (Cat# 102-15185) in HepG2 cell line lysates (35ug/lane). FEN1(arrow) was detected using the purified Pab.
	Western blot analysis of FEN1 (arrow) using rabbit polyclonal FEN1 Antibody (Center) (Cat# 102-15185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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