Anti-FGFR2 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P21802
Gene Id	2263
Gene Symbols	FGFR2
Protein Name / Synonyms	FGFR2
Clonality	Polyclonal
Preparation	This FGFR2 antibody is generated from rabbits immunized with a his tag recombinant protein of human FGFR2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in FGFR2 gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.

Specificity

Other Names

FGFR2; BEK; KGFR; KSAM; Fibroblast growth factor receptor 2; K-sam; Keratinocyte growth factor receptor; CD_antigen=CD332; Flags: Precursor

NCBI Accession #

NP_000132.3;NP_001138385.1;NP_001138386.1;NP_001138387.1;NP_001138388.1;NP_001138389.1;NP_001138390.1;NP_001138391.1;NP_075259.4;NP_075418.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92025

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of FGFR2 (arrow) using rabbit polyclonal FGFR2 Antibody (Cat# 102-17500). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR2 gene (Lane 2) (Origene Technologies).
	Western blot analysis of FGFR2 Antibody (Cat# 102-17500) in NCI-H460 cell line lysates (35ug/lane). SFGFR2 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma with FGFR2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of FGFR2 Antibody(Cat# 102-17500) with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	TLR4 Antibody (Center) (Cat# 102-17500) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.