Anti-FGFR4 (N-term) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P22455
Gene Id	2264
Gene Symbols	FGFR4
Protein Name / Synonyms	FGFR4 (N-term)
Clonality	Polyclonal
Preparation	This FGFR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human FGFR4.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

FGFR4 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of the gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.

Specificity

Other Names

FGFR4; JTK2; TKF; Fibroblast growth factor receptor 4; CD_antigen=CD334; Flags: Precursor

NCBI Accession #

NP_002002.3;NP_075252.2;NP_998812.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

87954

Recommended Dilutions

WB: 1:1000; IF: 1:200; FC: 1:10-50; IHC: 1:50-100

	Western blot analysis of lysates from SH-SY5Y 293, Raji cell line (from left to right), using FGFR4 Antibody (N-term) (Cat# 102-17502). 102-17502 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	Fluorescent confocal image of HeLa cells stained with FGFR4 (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-17502 FGFR4 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the FGFR4 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000160867).
	FGFR4 Antibody (E39) (Cat# 102-17502) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FGFR4 antibody detected the FGFR4 protein (arrow).
	Flow cytometric analysis of WiDr cells using FGFR4 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.