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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 9457 |
| Protein Name / Synonyms | FHL5 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ACT antibody, FLJ33049 antibody, KIAA0776 antibody, RP3-393D12.2 antibody, dJ393D12.2 antibody, FHL5 antibody, FHL-5 antibody, LIM protein ACT antibody, activator of cAMP-responsive element modulator (CREM) in testis antibody, activator of CREM in testis antibody, activator of cAMP-responsive element modulator in testis antibody, 1700027G07Rik antibody, four and a half LIM domains protein 5 antibody, four and a half LIM domains 5 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 226 of FHL5 (Uniprot ID# Q5TD97)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5(107-10663) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5(107-10663) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: Raji
12 % SDS PAGE
107-10663 diluted at 1:1000
A: 293T
B: Raji
12 % SDS PAGE
107-10663 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.