Anti-Flightless I (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	2314
Protein Name / Synonyms	Flightless I, Internal
Concentration (lot specific)	0.88 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLI antibody, FLIL antibody, Fli1 antibody, MGC39265 antibody, FLII antibody, protein flightless-1 homolog antibody, flightless I homolog (Drosophila) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

145 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 435 and 778 of Flightless I (Uniprot ID# Q13045)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I(107-10598) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I(107-10598) antibody at 1:200 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10598 diluted at 1:1000

Sample(30 ug whole cell lysate)
A:Raji
5% SDS PAGE
107-10598 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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