RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-FLNA Antibody

Catalog #: 144-61518

Phospho-FLNA-S2152 pAb

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P21333
Gene Id
2316
Gene Symbols
FLNA
Protein Name / Synonyms
FLNA, ABP-280, ABPX, CSBS, CVD1, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD, filamin-A
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
280kDa
Observed MW
300kDa

Immunogen

A synthetic phosphorylated peptide around S2152 of human FLNA (NP_001104026.1).

Modification

Phospho S2152

Storage

-20°C

Images

Western blot analysis of extracts of HeLa and C6 cells, using Phospho-FLNA-S2152 antibody (144-61518) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour .C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - Phospho-FLNA-S2152 antibody (144-61518)

Expiration:
12 months from the date of shipment when stored properly.