Anti-Flotillin 2 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	2319
Protein Name / Synonyms	Flotillin 2, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ECS-1 antibody, ECS1 antibody, ESA antibody, ESA1 antibody, M17S1 antibody, FLOT2 antibody, membrane component chromosome 17 surface marker 1 antibody, flotillin-2 antibody, epidermal surface antigen antibody, membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody, Flotillin 2 (epidermal surface antigen 1) antibody, flotillin 2 antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

47 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 365 and 428 of Flotillin 2 (Uniprot ID# Q14254)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
10 % SDS-PAGE
Flotillin 2 antibody [C3], C-term (107-10201) dilution: 1:1000

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
10 % SDS-PAGE
Flotillin 2 antibody [C3], C-term (107-10201) dilution: 1:1000

Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10 % SDS-PAGE, and blotted with Flotillin 2 antibody (107-10201) diluted by 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 (107-10201) antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Lung CA, using Flotillin-2 (107-10201) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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