Rabbit Anti-Human FOXA2 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9Y261 |
| Gene Id | 3170 |
| Gene Symbols | FOXA2 |
| Protein Name / Synonyms | FOXA2 (Center) |
| Clonality | Polyclonal |
| Preparation | This FOXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the Central region of human FOXA2. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
FOXA2; HNF3B; TCF3B; Hepatocyte nuclear factor 3-beta; Forkhead box protein A2; Transcription factor 3B
NCBI Accession #
NP_068556.2;NP_710141.1
Other Accession #
P32182; P35583; NP_710141.1; NP_068556.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
48306
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | FOXA2 Antibody (Center) (Cat# 102-10952) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FOXA2 antibody detected the FOXA2 protein (arrow). |
 | FOXA2 Antibody (Center) (Cat# 102-10952)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXA2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Fluorescent confocal image of A549 cell stained with FOXA2 Antibody (Center)(Cat# 102-10952).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXA2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXA2 immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.