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Anti-FREM2 Antibody
Catalog #: 144-61307
FREM2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q5SZK8 |
| Gene Id | 341640 |
| Gene Symbols | FREM2 |
| Protein Name / Synonyms | FREM2, FRAS1-related extracellular matrix protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
295kDa/351kDa
Observed MW
351kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 47-300 of human FREM2 (NP_997244.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FREM2 antibody (144-61307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 150s.
Expiration:
12 months from the date of shipment when stored properly.