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Anti-Fumarate hydratase Antibody
Catalog #: 107-10875
Fumarate hydratase antibody, 0.9 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 2271 |
| Protein Name / Synonyms | Fumarate hydratase |
| Concentration (lot specific) | 0.9 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
HLRCC antibody, LRCC antibody, MCL antibody, MCUL1 antibody, FH antibody, fumarase antibody, fumarate hydratase, mitochondrial antibody, fumarate hydratase antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
55 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 195 and 473 of fumarate hydratase (Uniprot ID# P07954)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: Fumarate hydratase protein stained by Fumarate hydratase antibody (107-10875) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: Fumarate hydratase protein stained by Fumarate hydratase antibody (107-10875) diluted at 1:500.
Blue: Hoechst 33342 staining.
Fumarate hydratase antibody detects FH protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
7.5% SDS-PAGE
Fumarate hydratase antibody (107-10875) dilution: 1:1000
A. 50 µg mouse liver lysate/extract
7.5% SDS-PAGE
Fumarate hydratase antibody (107-10875) dilution: 1:1000
Fumarate hydratase antibody detects Fumarate hydratase protein by Western blot analysis.
A.50 µg rat liver lysate/extract
10 % SDS-PAGE
Fumarate hydratase antibody (107-10875) dilution: 1:5000
A.50 µg rat liver lysate/extract
10 % SDS-PAGE
Fumarate hydratase antibody (107-10875) dilution: 1:5000
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (107-10875) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2
10% SDS PAGE
107-10875 diluted at 1:1000
A: HeLa
B: Hep G2
10% SDS PAGE
107-10875 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.