Anti-GABPB1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	2553
Protein Name / Synonyms	GABPB1
Concentration (lot specific)	0.63 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

BABPB2 antibody, E4TF1 antibody, E4TF1-47 antibody, E4TF1-53 antibody, E4TF1B antibody, GABPB antibody, GABPB2 antibody, NRF2B1 antibody, NRF2B2 antibody, GABPB1 antibody, transcription factor E4TF1-53 antibody, GABP subunit beta-2 antibody, nuclear respiratory factor 2 antibody, GABPB-1 antibody, GA-binding protein subunit beta-1 antibody, transcription factor E4TF1-47 antibody, "GA binding protein transcription factor, beta subunit 1 antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

42 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 395 of GABPB1 (Uniprot ID# Q06547)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GABPB1(107-10384) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using GABP-beta1(107-10384) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
107-10384 diluted at 1:5000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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