Synonyms
GAD65 antibody, MGC161605 antibody, MGC161607 antibody, GAD2 antibody, 65 kDa glutamic acid decarboxylase antibody, glutamate decarboxylase 65 kDa isoform antibody, GAD-65 antibody, glutamate decarboxylase 2 antibody, Glutamate decarboxylase-2 (pancreas) antibody, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
65 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 465 and 558 of GAD65 (Uniprot ID# Q05329)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
GAD65 antibody [C2C3], C-term (107-10202) dilution: 1:500.

Sample (10 ug of whole cell lysate)
A: rat brain
7.5% SDS PAGE
107-10202 diluted at 1:10000

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
107-10202 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.