Anti-GAD67 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	2571
Protein Name / Synonyms	GAD67
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ45882 antibody, GAD antibody, SCP antibody, GAD1 antibody, GAD-67 antibody, glutamate decarboxylase 67 kDa isoform antibody, glutamate decarboxylase 1 antibody, 67 kDa glutamic acid decarboxylase antibody, glutamate decarboxylase 1 (brain, 67kDa) antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

67 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67 (Uniprot ID# Q99259)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
GAD67 antibody (107-10298) dilution: 1:500.

GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 30 µg SK-N-SH whole cell lysate/extract
B. 30 µg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500

GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:5000

GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 50 µg mouse brian lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500

GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500

GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: GAD67-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min.
Green: GAD67 protein stained by GAD67 antibody (107-10298) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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