Anti-GALNS Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	2588
Protein Name / Synonyms	GALNS
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ17434 antibody, FLJ42844 antibody, FLJ98217 antibody, GALNAC6S antibody, GAS antibody, MPS4A antibody, GALNS antibody, galactose-6-sulfate sulfatase antibody, chondroitinase antibody, N-acetylgalactosamine-6-sulfate sulfatase antibody, galNAc6S sulfatase antibody, chondroitinsulfatase antibody, N-acetylgalactosamine-6-sulfatase antibody, galactosamine (N-acetyl)-6-sulfate sulfatase antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

58 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 20 and 259 of GALNS

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS(107-10906) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:500.

GALNS antibody detects GALNS protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
GALNS antibody (107-10906) dilution: 1:1000

GALNS antibody detects GALNS protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
GALNS antibody (107-10906) dilution: 1:500

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS(107-10906) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
107-10906 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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