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Anti-GALNT2 Antibody
Catalog #: 107-10427
GALNT2 antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 2590 |
| Protein Name / Synonyms | GALNT2 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
GalNAc-T2 antibody, GALNT2 antibody, polypeptide GalNAc transferase 2 antibody, UDP-GalNAc transferase 2 antibody, polypeptide N-acetylgalactosaminyltransferase 2 antibody, pp-GaNTase 2 antibody, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2 antibody, protein-UDP acetylgalactosaminyltransferase 2 antibody, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
65 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 55 and 369 of GALNT2 (Uniprot ID# Q10471)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
GALNT2 antibody [N1C1] detects GALNT2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GALNT2 protein stained by GALNT2 antibody [N1C1] (107-10427) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GALNT2 protein stained by GALNT2 antibody [N1C1] (107-10427) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded Colon ca, using GALNT2(107-10427) antibody at 1:250 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse white adipose tissue
7.5% SDS PAGE
107-10427 diluted at 1:1000
A: Mouse white adipose tissue
7.5% SDS PAGE
107-10427 diluted at 1:1000
GALNT2 antibody detects GALNT2 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
GALNT2 antibody (107-10427) dilution: 1:1000
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
GALNT2 antibody (107-10427) dilution: 1:1000
Sample(30 ug whole cell lysate)
A:MOLT4
7.5% SDS PAGE
107-10427 diluted at 1:500
A:MOLT4
7.5% SDS PAGE
107-10427 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.