Anti-GAPDH Antibody

Product Description

Specifications

Size	0.2 mg
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Mouse
Protein Name / Synonyms	GAPDH
Concentration (lot specific)	1.0mg/ml
Recommended Applications	Immunohistology - Frozen, ELISA, Immunofluorescence, Immunoprecipitation, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Specificity

This product recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36kD multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.

GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:

Purified IgG - liquid

Formulation:

Phosphate buffered saline

Format Type:

Purified

Preparation

Immunogen:

Human cardiac muscle GADPH

Preservative:

0.09% Sodium Azide (NaN₃)

This product

Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.

Expiration:

12 months from the date of shipment when stored properly.

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