Synonyms
GAR22 antibody, MGC17243 antibody, GAS2L1 antibody, GAS2-like protein 1 antibody, growth arrest-specific protein 2-like 1 antibody, GAS2-related protein on chromosome 22 antibody, growth arrest-specific 2 like 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
73 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 71 and 355 of GAS2L1 (Uniprot ID# Q99501)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using GAS2L1(107-10592) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using GAS2L1(107-10592) antibody at 1:100 dilution.

GAS2L1 antibody detects GAS2L1 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg A375 whole cell lysate/extract
10 % SDS-PAGE
GAS2L1 antibody (107-10592) dilution: 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
107-10592 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.