Anti-GCAT (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O75600
Gene Id	23464
Gene Symbols	GCAT
Protein Name / Synonyms	GCAT (Center)
Clonality	Polyclonal
Preparation	This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

Specificity

Predicted Reactivity

Bovine

Other Names

GCAT; KBL; 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; Aminoacetone synthase; Glycine acetyltransferase

NCBI Accession #

NP_001165161.1;NP_055106.1

Other Accession #

O88986; Q0P5L8

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

45285

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of GCAT antibody (Center) (Cat# 102-17387) in mouse liver tissue lysates (35ug/lane). GCAT (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (Center) (Cat# 102-17387). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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