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Anti-GCP2 (N-term) Antibody
Catalog #: 107-10332
GCP2 antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 10844 |
| Protein Name / Synonyms | GCP2, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
GCP2 antibody, MGC138162 antibody, SPBC97 antibody, Spc97p antibody, TUBGCP2 antibody, gamma-ring complex protein 103 kDa antibody, hGrip103 antibody, spindle pole body protein Spc97 homolog antibody, h103p antibody, GCP-2 antibody, gamma-tubulin complex protein 2 antibody, gamma-tubulin complex component 2 antibody, hGCP2 antibody, hSpc97 antibody, "tubulin, gamma complex associated protein 2 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
103 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 226 of GCP2 (Uniprot ID# Q9BSJ2)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
GCP2 antibody [N1N2], N-term detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term (107-10332) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term (107-10332) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Immunohistochemical analysis of paraffin-embedded Hepatoma, using GCP2(107-10332) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
107-10332 diluted at 1:2000
A: NIH-3T3
7.5% SDS PAGE
107-10332 diluted at 1:2000
Sample (30 ug of whole cell lysate)
A: A431
B: Hela
7.5% SDS PAGE
107-10332 diluted at 1:1000
A: A431
B: Hela
7.5% SDS PAGE
107-10332 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.