Anti-GDNF (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P39905
Gene Id	2668
Gene Symbols	GDNF
Protein Name / Synonyms	GDNF (N-term)
Clonality	Polyclonal
Preparation	This GDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of human GDNF.
Concentration (lot specific)	0.380 mg/ml
Recommended Applications	IHC-P, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described.

Specificity

Other Names

GDNF; Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor

NCBI Accession #

NP_000505.1;NP_001177397.1;NP_001177398.1;NP_001265027.1;NP_954701.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

23720

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of GDNF Antibody (N-term) (Cat# 102-17982) in mouse NIH-3T3 cell line lysates (35ug/lane). GDNF (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	GDNF Antibody (N-term) (Cat# 102-17982) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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