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ISO 13485:2016 cGMP
Anti-GFP (201-220aa) Antibody
Catalog #: 130-11011
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1000 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Aequorea victoria |
| Host Species | Rabbit |
| Accession Number | P42212 |
| Gene Symbols | GFP |
| Protein Name / Synonyms | GFP (201-220aa) |
| Clonality | Polyclonal |
| Immunogen | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen GFP (201-220aa): YLSTQSALSKDPNEKRDHMVC. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Immunogen Sequence | YLSTQSALSKDPNEKRDHMVC |
| Recommended Applications | ELISA (recommended work dilution = 1:20, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
Western Blot
130-11011 (pAb): 1:2000
10 % SDS-PAGE
Goat anti-rabbit IgG-HRP: 1:10,000
Lane: M: protein marker 5 µl
- GFP/Hela cell lysate 10 µl
- Hela cell lysate 10 µl
- GFP/CHO cell lysate 10 µl
Simple Western
Lane 1: Biot. Ladder
2: CHO
3: CHO/GFP
4: Hela/GFP
5: Hela
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.