Synonyms
CD224 antibody, D22S672 antibody, D22S732 antibody, GGT antibody, GGT 1 antibody, GTG antibody, MGC96892 antibody, MGC96904 antibody, MGC96963 antibody, GGT1 antibody, gamma-glutamyl transpeptidase antibody, gamma-glutamyltranspeptidase 1 antibody, glutamyl transpeptidase antibody, gamma-glutamyltransferase 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 226 of GGT1 (Uniprot ID# P19440)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using GGT1(107-10248) antibody at 1:100 dilution.

GGT1 antibody detects GGT1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
GGT1 antibody (107-10248) dilution: 1:1000

Sample(30 µg of whole cell lysate)
A:MOLT4
7.5% SDS PAGE
107-10248 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.