Anti-GLK (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8IVH8
Gene Id	8491
Gene Symbols	MAP4K3
Protein Name / Synonyms	GLK (C-term)
Clonality	Polyclonal
Preparation	This GLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-453 amino acids from the C-terminal region of human GLK.
Concentration (lot specific)	1.050 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

GLK is a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).

Specificity

Other Names

MAP4K3; RAB8IPL1; Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; MAPK/ERK kinase kinase kinase 3

NCBI Accession #

NP_001257354.1;NP_003609.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

101316

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-GLK Pab (Cat# 102-17748) in Hela cell lysate. Dilution for anti-GLK was 1:100; dilution for secondary goat anti-rabbit-HRP was 1:7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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