Anti-Glucagon (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P01275
Gene Id	2641
Gene Symbols	GCG
Protein Name / Synonyms	Glucagon (C-term)
Clonality	Polyclonal
Preparation	This Glucagon antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the C-terminal region of human Glucagon.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Apoptosis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Glucagon is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.

Specificity

Other Names

GCG; Glucagon; Glicentin; Glicentin-related polypeptide; Oxyntomodulin; Glucagon-like peptide 1; Incretin hormone; Glucagon-like peptide 1(7-37); Glucagon-like peptide 1(7-36); Glucagon-like peptide 2

NCBI Accession #

NP_002045.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

20909

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of Glucagon Antibody (C-term) (Cat# 102-16946) in Jurkat cell line lysates (35ug/lane). GCG (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human colon carcinoma with Glucagon Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of Glucagon Antibody (C-term) (Cat# 102-16946) with pancreas tissue followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	Flow cytometric analysis of WiDr cells using Glucagon Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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