Synonyms
GKP2 antibody, GKTA antibody, GK2 antibody, ATP:glycerol 3-phosphotransferase 2 antibody, glycerol kinase pseudogene 2 antibody, glycerol kinase 2 antibody, glycerol kinase testis specific 2 antibody, "glycerol kinase, testis specific 2 antibody", GK 2 antibody, glycerokinase 2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 67 and 338 of Glycerol kinase 2 (Uniprot ID# Q14410)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Glycerol kinase 2(107-10389) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using glycerol kinase 2(107-10389) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
glycerol kinase 2 antibody
107-10389 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.