3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Glyoxalase I Antibody
Catalog #: 107-10560
Glyoxalase I antibody [N1C3], 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 2739 |
| Protein Name / Synonyms | Glyoxalase I |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
GLOD1 antibody, GLYI antibody, GLO1 antibody, aldoketomutase antibody, glyoxalase domain containing 1 antibody, lactoylglutathione lyase antibody, S-D-lactoylglutathione methylglyoxal lyase antibody, lactoyl glutathione lyase antibody, methylglyoxalase antibody, ketone-aldehyde mutase antibody, glx I antibody, glyoxalase I antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
21 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 184 of Glyoxalase I (Uniprot ID# Q04760)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Glyoxalase I antibody [N1C3] detects GLO1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GLO1 protein stained by Glyoxalase I antibody [N1C3] (107-10560) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GLO1 protein stained by Glyoxalase I antibody [N1C3] (107-10560) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10560 diluted at 1:1000
A: Mouse brain
12% SDS PAGE
107-10560 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded OVCA, using GLO1(107-10560) antibody at 1:100 dilution.
Sample(30µg whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
12% SDS PAGE
107-10560 diluted at 1:1000
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
12% SDS PAGE
107-10560 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.