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Anti-GM-CSF/CSF2 Antibody, Biotinylated
Catalog #: RB-IP-01-129B-50
Mouse anti-GM-CSF/CSF2 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | NM_000758 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was whole expressing mouse GM-CSF. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells deriveded from the immunized mouse. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-4, 000), Western Blotting( recommended work dilution= 1-2 µg/ml) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Granulocyte-macrophage colony-stimulating factor (GM-CSF), is a protein secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts. It is a 22 kDa monomeric hematopoietic cytokine. GM-CSF-stimulated monocytes exhibit several anti-tumor behaviors. GM-CSF enhances the ability of macrophages to present antigen and initiate an immune response. It also stimulates monocytes to secrete large amounts of a soluble VEGF receptor, which binds to and inactivates VEGF.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody specifically detected protein derived from human. It also recognizes tested samples containing GM-CSF in both ELISA and WB. This antibody shows no cross-reactivity with other tested cytokines.
References
- Armstrong, C. A., Botella, R., Galloway, T. H., Murray, N., Kramp, J. M., and Song, I. S. 1996. Antitumor effects of granulocyte-macrophage colony-stimulating factor production by melanoma cells. Cancer Research 56.2191-8.
Expiration:
12 months from the date of shipment when stored properly.