GOLPH2 antibody [C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 51280 |
| Protein Name / Synonyms | GOLPH2, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
C9orf155 antibody, FLJ22634 antibody, FLJ23608 antibody, GOLPH2 antibody, GP73 antibody, PSEC0257 antibody, bA379P1.3 antibody, GOLM1 antibody, golgi phosphoprotein 2 antibody, golgi membrane protein GP73 antibody, Golgi membrane protein 1 antibody, "golgi protein, 73-kD antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
45 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 339 and 401 of GOLPH2 (Uniprot ID# Q8NBJ4)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2(107-10087) antibody at 1:200 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2(107-10087) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled at 1:2500.
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
GOLPH2 antibody [C3], C-term (107-10087) dilution: 1:500.
Sample: Paraffin-embedded human colon carcinoma.
GOLPH2 antibody [C3], C-term (107-10087) dilution: 1:500.
GOLPH2 antibody detects GOLPH2 protein by western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg HeLa membrane fraction lysate/extract
10 % SDS-PAGE
GOLPH2 antibody (107-10087) dilution: 1:500
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg HeLa membrane fraction lysate/extract
10 % SDS-PAGE
GOLPH2 antibody (107-10087) dilution: 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.