Anti-GORASP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9BQQ3
Gene Id	64689
Gene Symbols	GORASP1
Protein Name / Synonyms	GORASP1, GOLPH5, GRASP65, P65, Golgi reassembly-stacking protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

23kDa/26kDa/46kDa

Observed MW

65kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GORASP1 (NP_114105.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GORASP1 antibody (144-06609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - GORASP1 antibody (144-06609)

Immunohistochemistry of paraffin-embedded human gastric cancer using GORASP1 antibody (144-06609) at dilution of 1:100 (40x lens).
Immunohistochemistry - GORASP1 antibody (144-06609)

Immunofluorescence analysis of A549 cells using GORASP1 antibody (144-06609). Blue: DAPI for nuclear staining.
Immunofluorescence - GORASP1 antibody (144-06609)

Expiration:

12 months from the date of shipment when stored properly.

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