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Anti-GOT1 (C-term) Antibody
Catalog #: 102-15249
Rabbit Anti-GOT1 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P17174 |
| Gene Id | 2805 |
| Gene Symbols | GOT1 |
| Protein Name / Synonyms | GOT1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-381 amino acids from the C-terminal region of human GOT1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Specificity
Predicted Reactivity
Rat
Other Names
GOT1; Aspartate aminotransferase, cytoplasmic; Glutamate oxaloacetate transaminase 1; Transaminase A
NCBI Accession #
NP_002070.1
Other Accession #
P13221; P05201
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
46248
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of GOT1 Antibody (C-term) (Cat# 102-15249) in mouse liver tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab. |
 | Western blot analysis of GOT1 (arrow) using rabbit polyclonal GOT1 Antibody (C-term) (Cat# 102-15249). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GOT1 gene. |
 | GOT1 Antibody (C-term) (Cat# 102-15249) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GOT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | GOT1 Antibody (C-term) (Cat# 102-15249) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.