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Anti-GPD2 Antibody
Catalog #: 144-06611
GPD2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P43304 |
| Gene Id | 2820 |
| Gene Symbols | GPD2 |
| Protein Name / Synonyms | GPD2, GDH2, GPDM, mGPDH, glycerol-3-phosphate dehydrogenase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
67kDa/80kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GPD2 antibody (144-06611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver injury using GPD2 Antibody (144-06611) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GPD2 Antibody (144-06611) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using GPD2 Antibody (144-06611) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.