Anti-GRB2 (Y209) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P62993
Gene Id	2885
Gene Symbols	GRB2
Protein Name / Synonyms	GRB2 (Y209)
Clonality	Polyclonal
Preparation	This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from human GRB2.
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway.

Specificity

Predicted Reactivity

Mouse, Rat, Chicken, Xenopus

Other Names

GRB2; ASH; Growth factor receptor-bound protein 2; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2

NCBI Accession #

NP_002077.1;NP_987102.1

Other Accession #

P62994; Q60631; Q07883; Q6GPJ9; P87379

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

25206

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of lysate from Ramos cell line, using GRB2 Antibody (pY209)(Cat# 102-16723). 102-16723 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8^°C (short-term); -20^°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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